The most common archived biospecimen in oncology, formalin-fixed paraffin-embedded (FFPE) tissue is considered a key source material for gene expression profiling of tumors using RNA sequencing (RNA-Seq). However, FFPE RNA is highly degraded and is therefore suboptimal for most currently ...
Read More »DBiT-seq – in-tissue barcoding for spatially revolved whole transcriptome sequencing at cellular level
Formalin-fixed paraffin-embedded (FFPE) tissues are the most abundant archivable specimens in clinical tissue banks, but unfortunately incompatible with single-cell level transcriptome sequencing due to RNA...
Read More »3′ MACE RNA-sequencing allows for transcriptome profiling in human tissue samples after long-term storage
Researchers at the University of Freiburg aimed to compare the potential of standard RNA-sequencing (RNA-Seq) and 3′ massive analysis of c-DNA ends (MACE) RNA-sequencing for the analysis of fresh...
Read More »Biological validation of RNA sequencing data from formalin-fixed paraffin-embedded primary melanomas
Initiatives such as The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) have generated high-quality, multi-platform molecular data from thousands of frozen tumor...
Read More »Caris Life Sciences Receives FDA Breakthrough Device Designation for RNA-Seq Based Companion Diagnostic Test
This is the first companion diagnostic to detect gene fusions across all solid tumors; Caris expects to submit for Pre-Market Approval later this year Caris Life Sciences, a leading innovator in molecular...
Read More »Cofactor Genomics launches Paragon, an RNA sequencing-based solid tumor immune profiling assay
Cofactor Genomics, a clinical RNA sequencing and translational assay developer, announced today the commercial launch of Paragon, a solid tumor immune-profiling assay requiring only two FFPE...
Read More »Upcoming Webinar – Comparative Analysis of FFPE-Derived RNA: A Novel Relationship Between RNA Quality and Sequencing Results
December 18, 2017, 11AM EST, 4PM GMT, 5PM CET, 8AM PST RNA derived from formalin fixed, paraffin embedded (FFPE) tissues are invaluable for gene expression analysis of a myriad of disease states. However, the process of fixing the tissue and embedding ...
Read More »An NGS assay to quantify biomarkers of the host immune response in FFPE tumor samples
OmniSeq Inc scientists have developed a next-generation sequencing assay to quantify biomarkers of the host immune response in formalin-fixed, paraffin-embedded (FFPE) tumor specimens. This assay aims to provide clinicians with a comprehensive characterization of the immunological tumor microenvironment as a ...
Read More »Cofactor Genomics Announces $18M Funding Round to Bring RNA to Precision Medicine
Cofactor Genomics, developer of a leading platform for RNA sequencing and expression analysis, today announced $18M in financing led by Menlo Ventures with major participation from existing investor Data Collective (DCVC) and new investor Ascension Ventures, and participation from existing ...
Read More »A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples
RNA-sequencing (RNA-seq) has emerged as one of the most sensitive tool for gene expression analysis. Among the library preparation methods available, the standard poly(A) + enrichment provides a comprehensive, detailed, and accurate view of polyadenylated RNAs. However, on samples of ...
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