Consistent Errors in First Strand cDNA Due to Random Hexamer Mispriming

January 7, 2014 Leave a comment 5,914 Views

Priming of random hexamers in cDNA synthesis is known to show sequence bias, but in addition it has been suggested recently that mismatches in random hexamer priming could be a cause of mismatches between the original RNA fragment and observed ...

RNA-Seq Blog Transcriptome Research & Industry News

Tag Archives: First Strand cDNA

Consistent Errors in First Strand cDNA Due to Random Hexamer Mispriming

Establishment of a hybridoma sequencing pipeline for sequencing of antibody variable regions

RNA-seq identifies stochastic RNA editing of synaptic genes in single neurons

NVIGEN pioneers breakthrough in high-resolution tissue RNA sequencing with magnetic nanoparticle capture

RNA folding studies by tRNA structure-seq inside peptide-rich droplets reveal roles of modified nucleosides at the origin of life

GiRAFR – gRNA detection and annotation in single-cell CRISPR screens

RNA sequencing reaveals novel intracellular signaling mechanisms that promote melanoma growth

AAV-Perturb-seq – adeno-associated virus (AAV)-mediated direct in vivo single-cell CRISPR screening