Spatially resolved transcriptomics gets increasing attention during the recent years. New protocols based on in situ sequencing or multiplexed single-molecule fluorescent in situ hybridization (sm-FISH) aim at capturing physical positions of single molecules. It enables investigation of expression...
Read More »The combination of WGS and RNA-Seq is superior to conventional diagnostic tests in multiple myeloma
The diagnosis and risk stratification of multiple myeloma (MM) is based on clinical and cytogenetic tests. Magnetic CD138 enrichment followed by interphase FISH (fluorescence in situ hybridisation) is the...
Read More »Probe-Seq – transcriptional profiling of specific cell types from heterogeneous tissue by RNA-based isolation
Recent transcriptional profiling technologies are uncovering previously-undefined cell populations and molecular markers at an unprecedented pace. While single cell RNA (scRNA) sequencing is an attractive...
Read More »SAVER – gene expression recovery for single-cell RNA sequencing
In single-cell RNA sequencing (scRNA-seq) studies, only a small fraction of the transcripts present in each cell are sequenced. This leads to unreliable...
Read More »Single cell transcriptomics – methods and applications
Traditionally, gene expression measurements were performed on “bulk” samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular ...
Read More »Sequencing localized RNA in single cells by FISH
from greenfluorescentblog by Gal Haimovich To celebrate the 2-year anniversary of this blog, lets talk about the new Science paper in which the authors claim to performs in situ single cell, single molecule RNA sequencing. So what’s the big deal? Well, ...
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