Tag Archives: Fold-Change

Using meta-analysis, high-dimensional transcriptome expression data from public repositories can be merged to make group comparisons that have not been considered in the original studies. Merging of...

Alignment-free RNA quantification tools have significantly increased the speed of RNA-seq analysis. However, it is unclear whether these state-of-the-art RNA-seq analysis pipelines can quantify...

Various biases affect high-throughput sequencing read counts. Contrary to the general assumption, researchers from Ludwig-Maximilians-University Munich show that bias does not always cancel out when fold changes are computed and that bias affects more than 20% of genes that are ...

In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence ...

Reliability and reproducibility are key metrics for gene expression assays. This report assesses the utility of the correlation coefficient in the analysis of reproducibility and reliability of gene expression data. The correlation coefficient alone is not sufficient to assess equality ...