Tag Archives: FPKM

The temporal and spatial-specific expression pattern of a transcript in multiple tissues and cell types can indicate key clues about its function. While several gene atlas available online as pre-computed databases for known gene models, it’s still challenging to obtain ...

Neonatal dried blood spots (DBS) are routinely collected on standard Guthrie cards for all-comprising national newborn screening programs for inborn errors of metabolism, hypothyroidism and other diseases. In Denmark, the Guthrie cards are stored at – 20 °C in the ...

from StatQuest It used to be when you did RNA-seq, you reported your results in RPKM (Reads Per Kilobase Million) or FPKM (Fragments Per Kilobase Million). However, TPM (Transcripts Per Kilobase Million) is now becoming quite popular. Since there seems ...

Single-cell RNA-seq is a promising technology with broad applications and discerning biological noise from technical noise is critical for correctly interpreting the data (Jaitin, et al., 2014). Recently, statistical methods are developed to model the technical noise from spike-in ERCC ...

This video tutorial shows how to clean and map RNA-Seq data to any genome in CoGe. This includes generating BAM files, quantitating reads per position across a genome, and generating FPKM for genes. These data can be kept private and ...