Don't Miss
  • MATQ_Drop – Multiple-Annealing-and-Tailing-based Quantitative scRNA-seq in Droplets
  • Featured RNA-Seq Job – Research Technician II – Single Cell RNA Sequencing
  • Singleron launches single cell eQTL analysis as part of their sequencing service
  • IMMUcan scDB – a fully integrated scRNA-seq database exclusively dedicated to human cancer
  • Correspondence analysis for dimension reduction, batch integration, and visualization of single-cell RNA-seq data
  • Mutant KRAS signaling drives alternative splicing in lung cancer cells
  • Single cell RNA-sequencing identifies cause for loss of smell in long COVID patients
  • ESPRESSO – robust discovery and quantification of transcript isoforms from error-prone long-read RNA-seq data
  • Single-cell RNA sequencing reveals how Huntington’s disease affects different neurons
  • PAIso-seq reveals how poly(A)-tail-mediated remodeling of maternal mRNA controls start of life

RNA-Seq BlogRNA-Seq Blog Transcriptome Research & Industry News

  • Home
  • News
    • Commentary
    • Headlines
    • Industry News
    • Grants / Funding
    • Information
    • Poll Results
    • Press Releases
    • Transcriptome Sequenced
  • Events
    • Conferences
    • Meetings
    • Webinars
    • Workshops
    • Projects
    • Local Events
  • Jobs
  • Technology
    • Methods
      • Workflows
      • RNA Extraction
      • Library Preparation
      • Spatial Transcriptomics
      • Statistical Analysis
      • Data Analysis
        • Analysis Pipelines
        • Annotation
        • Clouding Platforms
        • Data Normalization
        • Data Sets
        • Data Visualization
        • Databases
        • Expression and Quantification
        • Other Tools
        • Pathway Analysis
        • SNP Detection
        • Spatial Analysis
        • Splicing and Junction Mapping
        • Statistical Analysis
        • Transcriptome Assembly Tools
        • Unspliced Mapping Tools
    • New Products
      • Our Advertisers
    • Patents
    • Presentations
    • Publications
    • Review Publications
    • Web Tools
  • Data Analysis
    • Analysis Pipelines
    • Annotation
    • Clouding Platforms
    • Data Normalization
    • Data Sets
    • Data Visualization
    • Databases
    • Expression and Quantification
    • Other Tools
    • Pathway Analysis
    • SNP Detection
    • Splicing and Junction Mapping
    • Statistical Analysis
    • Transcriptome Assembly Tools
  • Blog
  • Reader Posts
  • Contact
    • Administration
    • Contribute News
    • Subscribe
    • Advertise
    • Request for RNA-Seq Services

Tag Archives: GAGE

Tutorial – RNA-seq differential expression & pathway analysis with Sailfish, DESeq2, GAGE, and Pathview

December 10, 2015 1 Comment 12,786 Views

rna-seq

Read More »

Stay Connected

Publications Trend

Recent RNA-SEQ Pubs

Mesenchymal stromal cells ameliorate diabetes-induced muscle atrophy through exosomes by enhancing AMPK/ULK1-mediated autophagy
28 January 2023
Multiple variation patterns of terpene synthases in 26 maize genomes
28 January 2023
Bacterial droplet-based single-cell RNA-seq reveals antibiotic-associated heterogeneous cellular states
28 January 2023
Network analysis of large-scale ImmGen and Tabula Muris datasets highlights metabolic diversity of tissue mononuclear phagocytes
28 January 2023
Melatonin activates mitochondrial unfolded protein response to preserve osteogenic potential of senescent BMSCs via upregulating PDI-6
28 January 2023
Identification of a tumour immune barrier in the HCC microenvironment that determines the efficacy of immunotherapy
28 January 2023
Chromatin landscape governing murine epidermal differentiation
28 January 2023
Single-cell RNA Sequencing Reveals Homogeneous Transcriptome Patterns and Low Variance in a Suspension CHO-K1 and an Adherent HEK293FT Cell Line in Culture Conditions
28 January 2023
Copper ions inhibit Streptococcus mutans-Veillonella parvula dual biofilm by activating Streptococcus mutans reactive nitrogen species
28 January 2023
Key circRNAs from goat: discovery, integrated regulatory network and their putative roles in the differentiation of intramuscular adipocytes
28 January 2023
Improved downstream functional analysis of single-cell RNA-sequence data using DGAN
28 January 2023
circRNA432 enhances the coelomocyte phagocytosis via regulating the miR-2008-ELMO1 axis in Vibrio splendidus-challenged Apostichopus japonicus
28 January 2023
Comparison between qPCR and RNA-seq reveals challenges of quantifying HLA expression
27 January 2023
Identification of Novel Quantitative Trait Loci for Culm Thickness of Rice Derived from Strong-Culm Landrace in Japan, Omachi
27 January 2023
Analyzing RNA-Protein Interactions by Cross-Link Rates and CLIP-seq Libraries
27 January 2023
Effect of matrine in MAC-T cells and their transcriptome analysis: A basic study
27 January 2023
RNA 3D Modeling with FARFAR2, Online
27 January 2023
The antimicrobial peptide LK2(6)A(L) exhibits anti-inflammatory activity by binding to the myeloid differentiation 2 domain and protects against LPS-induced acute lung injury in mice
27 January 2023
Time series transcriptome analysis implicates the circadian clock in the <em>Drosophila melanogaster</em> female's response to sex peptide
27 January 2023
GENETIC DELETION OF TRANSLOCATOR PROTEIN EXACERBATES POST-SEPSIS SYNDROME WITH ACTIVATION OF THE C1Q PATHWAY IN SEPTIC MOUSE MODEL
27 January 2023
Transient developmental exposure to low doses of bisphenol F negatively affects neurogliogenesis and olfactory behaviour in adult mice
27 January 2023
Identification and validation of a prognostic signature of autophagy, apoptosis and pyroptosis-related genes for head and neck squamous cell carcinoma: to imply therapeutic choices of HPV negative patients
27 January 2023
Comparative transcriptomic analysis of the gene expression and underlying molecular mechanism of submergence stress response in orchardgrass roots
27 January 2023
A novel non-sense variant in the OFD1 gene caused Joubert syndrome
27 January 2023
Influence of RNA-Seq library construction, sampling methods, and tissue harvesting time on gene expression estimation
27 January 2023
Effect of salinity on ccmfn gene RNA editing of mitochondria in wild barley and uncommon types of RNA editing
27 January 2023
Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
27 January 2023
Immunomodulatory effects and mechanisms of Tiepishihu Xiyangshen granules on cyclophosphamide induced immuno-suppression via TLR4/MAPKs and PI3K/AKT/FOXO3a signal pathways
27 January 2023
Comparative transcriptomics in alternate bearing cultivar Dashehari reveals the genetic model of flowering in mango
27 January 2023
Molecular phenotyping of single pancreatic islet leader beta cells by "Flash-Seq"
27 January 2023
Single-cell RNA sequencing reveals the molecular features of peripheral blood immune cells in children, adults and centenarians
27 January 2023
Immune response gene 1 deficiency aggravates high fat diet-induced nonalcoholic fatty liver disease via promotion of redox-sensitive AKT suppression
27 January 2023
Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level
27 January 2023
Severe hematopoietic stem cell inflammation compromises chronic granulomatous disease gene therapy
27 January 2023
Overcoming the challenges associated with identification of deep intronic variants by whole genome sequencing
27 January 2023
The endothelial-enriched lncRNA LINC00607 mediates angiogenic function
26 January 2023
The potential role of N6-methyladenosine modification of LncRNAs in contributing to the pathogenesis of chronic glomerulonephritis
26 January 2023
The Transcriptional Response to Lung-Targeting Lipid Nanoparticles <em>in Vivo</em>
26 January 2023
Comparative transcriptome analysis reveals the biocontrol mechanism of Bacillus velezensis E68 against Fusarium graminearum DAOMC 180378, the causal agent of Fusarium head blight
26 January 2023
Intron-capture RNA-seq reveals the landscape of intronic RNAs in Arabidopsis
26 January 2023
MLL4 Regulates the Progression of Non-Small-Cell Lung Cancer by Regulating the PI3K/AKT/SOX2 Axis
26 January 2023
Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance
26 January 2023
Hepatitis D virus interferes with hepatitis B virus RNA production via interferon-dependent and -independent mechanisms
26 January 2023
A novel circular RNA circNLRP3 alleviated ricin toxin-induced TNF-α production through sponging miR-221-5p
26 January 2023
Genome-wide characterization, phylogenetic and expression analysis of histone gene family in cucumber (Cucumis sativus L.)
26 January 2023
Effect and mechanism of terahertz irradiation in repairing spinal cord injury in mice
26 January 2023
Cross center single-cell RNA sequencing study of the immune microenvironment in rapid progressing multiple myeloma
26 January 2023
Transcriptomics and co-expression network analysis revealing candidate genes for the laccase activity of Trametes gibbosa
26 January 2023
H3K4me3 mediates uterine leiomyoma pathogenesis via neuronal processes, synapsis components, proliferation, and Wnt/β-catenin and TGF-β pathways
26 January 2023
Molecular signatures of inherited and acquired sporadic late onset nemaline myopathies
26 January 2023

News in Pictures

rna-seq
Fred Hutch
singleron
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq

recent visitors

Subscribe to our Newsletter


Email Frequency

RNA-Seq Products & Services

Most Shared Posts

  • eTAM-seq – evolved TadA-assisted N6-methyladenosine sequencing
    Google +1 logo   Twitter logo   Facebook logo
  • IMMUcan scDB – a fully integrated scRNA-seq database exclusively dedicated to human cancer
    Google +1 logo   Twitter logo   Facebook logo
  • An application of RNA sequencing toward mRNA based nanotherapeutics against SARS-CoV-2 infections
    Google +1 logo   Twitter logo   Facebook logo
  • Total RNA-Seq a favorable alternative to metagenomics for taxonomic ID of microbial communities
    Google +1 logo   Twitter logo   Facebook logo
  • Exploring the epitranscriptome by native RNA sequencing
    Google +1 logo   Twitter logo   Facebook logo
  • Popular
  • Recent
  • Comments
  • rna-seq

    RPKM, FPKM and TPM, clearly explained

    July 22, 2015
  • rna-seq

    How to Analyze RNA-Seq Data?

    October 10, 2016
  • rna-seq

    Visualization of nucleotide substitutions in the (micro)transcriptome

    August 13, 2014
  • rna-seq

    Comparison of TMM (edgeR), RLE (DESeq2), and MRN Normalization Methods

    October 6, 2016
  • rna-seq

    An RNA-Seq QC Overview

    October 4, 2016
  • rna-seq

    MATQ_Drop – Multiple-Annealing-and-Tailing-based Quantitative scRNA-seq in Droplets

    2 days ago
  • Fred Hutch

    Featured RNA-Seq Job – Research Technician II – Single Cell RNA Sequencing

    2 days ago
  • rna-seq

    IMMUcan scDB – a fully integrated scRNA-seq database exclusively dedicated to human cancer

    3 days ago
  • rna-seq

    Correspondence analysis for dimension reduction, batch integration, and visualization of single-cell RNA-seq data

    3 days ago
  • rna-seq

    Mutant KRAS signaling drives alternative splicing in lung cancer cells

    4 days ago
  • Navi: Hi, I am new in RNA sequence analysis, I want to compare the...
  • Peter Lee: The industry already has next-generation sequencing (NGS) an...
  • Wei: Thanks for the shoutout! Hope PlaqView will be a valuable to...
  • Lilly: I thinks both of your explanations make sense. As you said,...
  • Kathi Gosche: I would like advertising rates, please....

Categories

Archives

What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

Recent News

  • rna-seq

    MATQ_Drop – Multiple-Annealing-and-Tailing-based Quantitative scRNA-seq in Droplets

    2 days ago
  • Fred Hutch

    Featured RNA-Seq Job – Research Technician II – Single Cell RNA Sequencing

    2 days ago
  • rna-seq

    IMMUcan scDB – a fully integrated scRNA-seq database exclusively dedicated to human cancer

    3 days ago
  • rna-seq

    Correspondence analysis for dimension reduction, batch integration, and visualization of single-cell RNA-seq data

    3 days ago
  • rna-seq

    Mutant KRAS signaling drives alternative splicing in lung cancer cells

    4 days ago
  • rna-seq

    Single cell RNA-sequencing identifies cause for loss of smell in long COVID patients

    4 days ago
  • rna-seq

    ESPRESSO – robust discovery and quantification of transcript isoforms from error-prone long-read RNA-seq data

    5 days ago

Subscribe to the RNA-Seq Blog


Email Frequency

Social Networking Pages


RNA-Seq
  • 8,146 Followers
  • 2,079 Fans
© Copyright 2009- , All Rights Reserved. | Designed by Go Big - Online Presence Creations