Analysis of differential gene expression by RNA sequencing (RNA-Seq) is frequently done using feature counts, i.e. the number of reads mapping to a gene. However, commonly used count algorithms (e.g. HTSeq) do not address the problem of reads aligning with ...
Read More »RNA-Rocket: An RNA-Seq Analysis Resource for Infectious Disease Research
RNA-Seq is a method for profiling transcription using high-throughput sequencing and is an important component of many research projects that wish to study transcript isoforms, condition specific expression, and transcriptional structure. The methods, tools, and technologies employed to perform RNA-Seq ...
Read More »Using Volcano Plots in R to Visualize Microarray and RNA-seq Results
This article originally appeared on Getting Genetics Done and graciously shared here by the author Stephen Turner. I’ve been asked a few times how to make a so-called volcano plot from gene expression results. A volcano plot typically plots some ...
Read More »