RNA-Seq data analysis has become vital to understanding gene structure and expression patterns in transcriptomic studies. A typical reference genome guided RNA-Seq workflow...
Read More »Potential pitfalls in analyzing and quantifying lowly-expressed genes and small RNAs with alignment-free pipelines
Alignment-free RNA quantification tools have significantly increased the speed of RNA-seq analysis. However, it is unclear whether these state-of-the-art RNA-seq analysis pipelines can quantify...
Read More »Step-by-step construction of gene co-expression networks from high-throughput RNA sequencing data
The rapid increase in the availability of transcriptomics data generated by RNA sequencing represents both a challenge and an opportunity for biologists without bioinformatics training. The challenge is handling, integrating, and interpreting these...
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