How to count multi-mapping reads?

September 19, 2017 Leave a comment 11,831 Views

RNA-Seq is currently used routinely, and it provides accurate information on gene transcription. However, the method cannot accurately estimate duplicated genes expression. Several strategies have been previously used (drop duplicated genes, distribute uniformly the reads, or estimate expression), but all ...

HTSeq – a Python framework to work with high-throughput sequencing data

October 31, 2014 Leave a comment 12,204 Views

A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard workflows, custom scripts are needed. Researchers at the European Molecular Biology Laboratory present HTSeq, a ...

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How to count multi-mapping reads?

HTSeq – a Python framework to work with high-throughput sequencing data

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Pan-cancer single-cell RNA-sequencing analysis reveals cancer cell states recur across tumor types

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Unearthing novel fusions as therapeutic targets in solid tumors using targeted RNA sequencing

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RNA Sequencing identifies potential targets for treating a common type of chronic, non-healing wound

Kyoto scientists use mathematics to extract clear signals from single-cell RNA sequencing data