The Human Cell Atlas is a large international collaborative effort to map all cell types of the human body. Single-cell RNA sequencing can generate high-quality data for the delivery of such an atlas. However, delays between fresh sample collection and ...
Read More »Probe-Seq – transcriptional profiling of specific cell types from heterogeneous tissue by RNA-based isolation
Recent transcriptional profiling technologies are uncovering previously-undefined cell populations and molecular markers at an unprecedented pace. While single cell RNA (scRNA) sequencing is an attractive...
Read More »A single-cell transcriptomic atlas of human neocortical development
UCLA researchers performed RNA sequencing on 40,000 cells to create a high-resolution single-cell gene expression atlas of developing human cortex, providing the first single-cell characterization of previously uncharacterized cell types, including human subplate neurons, comparisons with bulk tissue, and systematic ...
Read More »Columbia University: Two open post-doc positions for RNA-seq/spatial analyses in human brain disease
The Center for Translational and Computational Neuroimmunology at Columbia University in New York, NY (https://www.columbiactcn.org) is seeking two talented post-docs to lead analyses of bulk, single-cell, and spatial transcriptomics data from human brain samples in the context of brain disease. ...
Read More »viscRNA-Seq (virus-inclusive single cell RNA-Seq) – probing host transcriptome together with intracellular viral RNA at the single cell level
Dengue and Zika viral infections affect millions of people annually and can be complicated by hemorrhage or neurological manifestations, respectively. However, a thorough understanding of the host response to these viruses is lacking...
Read More »Buliding a Human from Transcriptional Profiles
The manual for human development is written in the genetic code of our DNA. Our genes encode the instructions to make specific types of molecules that are needed to either build the human body or to control its day-to-day operations. ...
Read More »Is RNA-Seq the best method to measure tissue specificity of gene expression?
One of the major properties of genes is their expression pattern. Notably, genes are often classified as tissue specific or housekeeping. This property is of interest to molecular evolution as an explanatory factor of, e.g. evolutionary rate, as well as ...
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