Tag Archives: illumina

The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP) – New database provides vast trove of genetic information on tiny single-celled organisms in the ocean

rna-seq

The ocean holds a bewildering array of microscopic life. Many of the smaller organisms are difficult to study or even to identify under a microscope. For this reason, scientists often look at the genes of these microbes to figure out ...

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RNA Sequencing Seminar – Karolinska Institutet and Illumina

rna-seq

for the Allergy, Immunology and Inflammation (Aii) doctoral education program Date: Wednesday 8 October 2014 Time: 13 –16 Venue: Westinrummet Alfred Nobels Allé 8, Huddinge (7th floor) Next generation sequencing (NGS) has led a revolution in life science research and ...

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A Comparison of Next Generation Sequencing Technologies for Transcriptome Assembly

De novo assembled transcriptomes, in combination with RNA-Seq, are powerful tools to explore gene sequence and expression level in organisms without reference genomes. Investigators must first choose which high throughput sequencing platforms will provide data most suitable for their experimental ...

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Open-access synthetic spike-in mRNA-seq data for cancer gene fusions

rna-seq

Oncogenic fusion genes underlie the mechanism of several common cancers. Next-generation sequencing based RNA-seq analyses have revealed an increasing number of recurrent fusions in a variety of cancers. However, absence of a publicly available gene-fusion focused RNA-seq data impedes comparative ...

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Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

rna-seq

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient ...

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TELP – a sensitive and versatile library construction method for next-generation sequencing

rna-seq

Next-generation sequencing has been widely used for the genome-wide profiling of histone modifications, transcription factor binding and gene expression through chromatin immunoprecipitated DNA sequencing (ChIP-seq) and cDNA sequencing (RNA-seq). Here, researchers from the Singapore Institute for Clinical Sciences describe a ...

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A method for extracting high-quality RNA from diverse plants for next-generation sequencing and gene expression analyses

To study expression in plants, high-quality RNA must be extracted in quantities sufficient for subsequent cDNA library construction. Field-based collections are often limited in quantity and quality of tissue and are typically preserved in RNAlater. Obtaining sufficient and high-quality yield ...

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Studying the Transcriptome with Next Generation Sequencing

rna-seq

Next-Generation Sequencing is enabling scientists to study the transcriptome in ways never before possible. During this session with Illumina Distinguished Scientist, Dr. Gary Schroth, you will learn how a variety of RNA-Seq methods can be used to profile subsets of ...

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Comprehensive Analysis of Alternative Splicing by Strand-Specific RNA-Seq

rna-seq

Digitalis purpurea (D. purpurea) is one of the most important medicinal plants and is well known in the treatment of heart failure because of the cardiac glycosides that are its main active compounds. However, in the absence of strand specific ...

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The concordance between RNA-seq and microarray data depends on chemical treatment and transcript abundance

rna-seq

The concordance of RNA-sequencing (RNA-seq) with microarrays for genome-wide analysis of differential gene expression has not been rigorously assessed using a range of chemical treatment conditions. Here a team led by researchers atuse at the US FDA comprehensive study design ...

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