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Tag Archives: Institut Pasteur

Well hidden cell type diversity and regulation finally revealed by whole-organism single-cell RNA-Seq

July 11, 2018 Leave a comment 2,090 Views

rna-seq

Despite its apparent simplicity – a tube-like body topped with tentacles –, the sea anemone is actually a highly complex creature. Scientists from the Institut Pasteur, in collaboration with the CNRS, have just discovered over a hundred different cell types in ...

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Schistosoma mansoni alter transcription of immunomodulatory gene products following in vivo praziquantel exposure
3 March 2021
Establishment of a Cuscuta campestris-mediated enrichment system for genomic and transcriptomic analyses of 'Candidatus Liberibacter asiaticus'
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The transcriptome characteritics of vestibular organs from delayed endolymphatic hydrops patients(Menieres disease)
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Effects of neonatal exposure to methoxychlor on corpus luteum in gilts: A transcriptomic analysis
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The type 2 asthma mediator IL-13 inhibits SARS-CoV-2 infection of bronchial epithelium
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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  • rna-seq

    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    7 hours ago
  • rna-seq

    RNA sequencing brings hope for endangered wild cats

    8 hours ago
  • rna-seq

    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

    1 day ago
  • rna-seq

    Uncovering the Anti-Myeloma Resistance Files

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  • rna-seq

    Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source

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    2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

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