Thanks to reduced cost of sequencing and library preparation, it is now possible to conduct a well-replicated RNA-Seq study for less than a few thousand dollars. However, if unforeseen problems arise, such as insufficient sequencing depth or batch effects, the ...
Read More »RNA-Seq Alignment and Visualization with IGB
Integrated Genome Browser (IGB) – RNA-Seq Alignment and Visualization (Focus on a Feature)
Read More »A Protocol for Visual Analysis of Alternative Splicing in RNA-Seq Data Using Integrated Genome Browser
Ultrahigh-throughput sequencing of cDNA (RNA-Seq) is an invaluable resource for investigating alternative splicing in an organism. Alternative splicing is a form of posttranscriptional regulation in which primary RNA transcripts from a single gene can be spliced in multiple ways leading ...
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