Though Illumina has largely dominated the RNA-Seq field, the simultaneous availability of Ion Torrent has left scientists wondering which platform is most effective for differential gene expression (DGE) analysis. Previous investigations of this question have typically used reference samples derived ...
Read More »Small RNA Library Construction for Exosomal RNA Sequencing on the Ion Torrent PGM™ and Ion S5™ System
Next-generation deep sequencing (NGS) technology represents a powerful and innovative approach to profile small RNA. Currently, there are a number of large-scale and benchtop sequencing platforms available on the market. Although each platform is relatively straightforward to operate, constructing cDNA ...
Read More »An optimized protocol for RNA-Seq on the Ion Proton sequencer
Previous studies compared running cost, time and other performance measures of popular sequencing platforms. However, comprehensive assessment of library construction and analysis protocols for Proton sequencing platform remains unexplored. Unlike Illumina sequencing platforms, Proton reads are heterogeneous in length and ...
Read More »Thermo Fisher Scientific Launches Ion Torrent AmpliSeq RNA Fusion Lung Cancer Research Panel
CARLSBAD, Calif.–(BUSINESS WIRE)–Today Thermo Fisher Scientific announced that it has released a next-generation sequencing (NGS) RNA panel and workflow that enables targeted sequencing of fusion transcripts for clinical research. The new Ion Torrent AmpliSeq RNA Fusion Lung Cancer Research Panel ...
Read More »RNA-Seq To ID Markers For Research on Neural Tissue Differentiation – ASHG 2014
Neural tissue differentiated and cultured from derived stem cells is expected to revolutionize the treatment of brain and spinal injuries and diseases. Critical for these cellular therapies is accurate control and monitoring of differentiation but current methods for such cell ...
Read More »QuantSeq 3’mRNA-Seq Library Prep Kit Adapted for Ion Torrent Users
Ion Torrent users can now also benefit from a fast, very strand-specific, and cost-effective library prep for sequencing of 3’ ends of the polyadenylated RNA. Today Lexogen announced the official release of QuantSeq 3’mRNA-Seq Library Prep Kit for Ion Torrent ...
Read More »The Evolution of Sequencing Technology
from Sciencemag.org posters
Read More »MaLTA – a method for simultaneous transcriptome assembly and quantification from Ion Torrent RNA-Seq data
High throughput RNA sequencing (RNA-Seq) can generate whole transcriptome information at the single transcript level providing a powerful tool with multiple interrelated applications including transcriptome reconstruction and quantification. The sequences of novel transcripts can be reconstructed from deep RNA-Seq data, ...
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