Tag Archives: Iso-Seq

Pacific Biosciences of California, Inc. today announced a new version of Sequel® Software (V5.1) and a new polymerase. Combined, these enhancements increase throughput and the overall...

The Isoform Sequencing (Iso - Seq™) method employs long reads to sequence transcript isoforms from the 5’ end to their poly - A tails, eliminating the need for transcript reconstruction and inference. These long reads result in complete, unambiguous...

A series of recent peer-reviewed scientific publications highlights the growing use of Single Molecule, Real-Time (SMRT®) Sequencing to annotate plant and animal genomes. These reports from the genomics community show that PacBio sequencing consistently detects more genes, alternative splicing events, ...

Alternative splicing (AS) is a major source of transcript and proteome diversity, but examining AS in species without well-annotated reference genomes remains difficult. Research on both human and mouse has demonstrated the advantages of using Iso-Seq™ data for isoform-level transcriptome ...

Short-read sequencing has enabled the de novo assembly of several individual human genomes, but with inherent limitations in characterizing repeat elements. Now, an international team led by researchers at USC have sequenced a Chinese individual HX1 by single-molecule real-time (SMRT) ...

Improvements in maize breeding are required to feed a growing population and will depend on a more complete understanding of global gene expression in the plant. In a new publication from Cold Spring Harbor Laboratory (CSHL), scientists produced the largest ...

The majority of human genes are alternatively spliced, making it possible for most genes to generate multiple proteins. Alternative splicing has been shown to play a role in human cancer, muscular dystrophy, Alzheimer’s, and many other diseases. Understanding these diseases ...

A new review article offers a nice overview of attempts to characterize the transcriptome of human stem cells using RNA-seq, the Iso-Seq™ method, and more. Kin Fai Au and Vittorio Sebastiano, scientists at the University of Iowa and Stanford University, ...

from the PacBio Blog With the recent launch of SMRT Analysis v2.2, we’re excited to introduce analysis software support for the new Iso-Seq™ method for sequencing full-length transcripts and gene isoforms, with no assembly required! Today we’ll take a deeper ...

MENLO PARK, Calif., March 31, 2014 (GLOBE NEWSWIRE) — Pacific Biosciences of California, Inc., (Nasdaq:PACB) provider of the PacBio® RS II, today announced the release of a software upgrade for its Single Molecule, Real-Time (SMRT®) DNA Sequencing platform. SMRT Analysis ...