Tag Archives: isoform abundance

Quantification of isoform abundance has been extensively studied at the mature-RNA level using RNA-seq but not at the level of precursor RNAs using nascent RNA sequencing. Researchers from Cold...

Understanding the regulation of gene expression, including transcription start site usage, alternative splicing, and polyadenylation, requires accurate quantification of expression levels down to the level of individual transcript isoforms. To comparatively evaluate the accuracy of the many methods that have ...

The advancement of RNA sequencing (RNA-seq) has provided an unprecedented opportunity to assess both the diversity and quantity of transcript isoforms in an mRNA transcriptome. In this paper, researchers from the University of Kentucky revisit the computational problem of transcript ...