Tag Archives: isoform expression

RNA-sequencing (RNA-Seq) has become a popular tool for transcriptome profiling in mammals. However, accurate estimation of allele-specific expression (ASE) based on alignments of reads to the reference genome is challenging, because it contains only one allele on a mosaic haploid ...

RNA-sequencing of single-cells enables characterization of transcriptional heterogeneity in seemingly homogenous cell populations. In this study, reseachers from the Karolinska Institute propose and apply a novel method, ISOform-Patterns (ISOP), based on mixture modeling, to characterize the expression patterns of pairs ...

RNA sequencing (RNA-Seq) allows an unbiased survey of the entire transcriptome in a high-throughput manner. A major application of RNA-Seq is to detect differential isoform expression across experimental conditions, which is of great biological interest due to its direct relevance ...

MIT 7.91J Foundations of Computational and Systems Biology, Spring 2014 View the complete course: http://ocw.mit.edu/7-91JS14 Instructor: David Gifford This lecture by Prof. David Gifford is about RNA-seq (RNA sequencing), a method of characterizing RNA molecules through next-generation sequencing. He begins with the ...

High-throughput RNA sequencing (RNA-Seq) enables quantification and identification of transcripts at single-base resolution. Recently, longer sequence reads become available thanks to the development of new types of sequencing technologies as well as improvements in chemical reagents for the Next Generation ...

Pre-mRNA splicing is a critical step in eukaryotic gene expression that contributes to proteomic, cellular and developmental complexity. Small nuclear (sn)RNAs are core spliceosomal components, however, the extent to which differential expression of snRNA isoforms regulates splicing is completely unknown. ...