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Tag Archives: junk dna

Genome regions once mislabeled ‘junk’ linked to heart failure

May 5, 2014 Leave a comment 5,498 Views

rna-seq

By Julia Evangelou Strait Large sections of the genome that were once referred to as “junk” DNA have been linked to human heart failure, according to research from Washington University School of Medicine in St. Louis. So-called junk DNA was ...

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RNA-Seq Transcriptome Analysis of the Liver and Brain of the Black Carp (Mylopharyngodon piceus) During Fasting
17 April 2021
Author Correction to: Single-cell RNA-seq reveals a concomitant delay in differentiation and cell cycle of aged hematopoietic stem cells
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Bioinformatics and survival analysis of glia maturation factor-γ in pan-cancers
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Decoding molecular markers and transcriptional circuitry of naive and primed states of human pluripotency
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    scGNN – a novel graph neural network framework for single-cell RNA-Seq analyses

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