Obtaining RNA-seq measurements involves a complex data analytical process with a large number of competing algorithms as options. There is much debate about which of these methods provides the best approach. Unfortunately, it is currently difficult to evaluate their performance ...
Read More »IIHG Intro to Sleuth for RNA-Seq
A brief introduction to the Sleuth R Shiny app for doing exploratory data analysis of your RNA-Seq data. This tutorial assumes that the data have been already quantified with kallisto and processed into a sleuth object with the sleuth r ...
Read More »kallisto – Near-optimal probabilistic RNA-seq quantification
Researchers from the University of California, Berkeley and the University of Iceland have developed kallisto, an RNA-seq quantification program that is two orders of magnitude faster than previous approaches and achieves similar accuracy. Kallisto pseudoaligns reads to a reference, producing ...
Read More »A sleuth for RNA-Seq
from Bits of DNA by Lior Pachter Today my student Harold Pimentel released the beta version of his new RNA-Seq analysis method and software program called sleuth. A sleuth for RNA-Seq begins with the quantification of samples with kallisto, and together a sleuth ...
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