Tag Archives: library preparation

The most common archived biospecimen in oncology, formalin-fixed paraffin-embedded (FFPE) tissue is considered a key source material for gene expression profiling of tumors using RNA sequencing (RNA-Seq). However, FFPE RNA is highly degraded and is therefore suboptimal for most currently ...

RNA sequencing (RNA-seq) is an integral tool in immunogenomics, allowing for interrogation of the transcriptome of a tumor and its microenvironment. Analytical methods to deconstruct the genomics data can then be applied to infer gene expression...

EMBL Course: Shift your DNA and RNA Sequencing Library Preparation into Hyper-Drive Course Overview The goal of this hands-on course is to teach the fundamentals of RNA and DNA library preparation for next generation sequencing applications. RNA depletion and enrichment strategies ...

RNA sequencing unlocks the mysteries hidden in the transcriptome. Whether your goal is gene expression analysis, gene fusion analysis, SNP analysis or miRNA expression analysis, achieving high-performance library preparation can prove essential to maximizing your discoveries. In this webinar, you ...

Next-generation sequencing technologies have revolutionized the study of small RNAs (sRNAs) on a genome-wide scale. However, classical sRNA library preparation methods introduce serious bias, mainly during adapter ligation steps. Several types...

Researchers from the Scripps Research Institute describe a method for making Illumina-compatible sequencing libraries from RNA. This protocol can be used for standard RNAseq analysis for detecting differentially expressed genes. In addition, this protocol is ideally suited for adapting to ...

Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same genomic coordinates using reference-based alignment. ...

Event status: Not started (Register) Date and time: Thursday, September 7, 2017 1:00 pm Eastern Daylight Time (New York, GMT-04:00) Duration: 1 hour Description: This webinar will address improvements in the library prep workflow for small RNA sequencing in serum ...

RNA sequencing (RNA-seq) has become an indispensable tool to identify disease associated transcriptional profiles and determine the molecular underpinnings of diseases. However, the broad adaptation of the methodology into the clinic is still hampered by inconsistent results from different RNA-seq ...

MicroRNAs (miRNAs) are ~22-nucleotide-long small non-coding RNAs that regulate the expression of protein-coding genes by base pairing to partially complementary target sites, preferentially located in the 3´ untranslated region (UTR) of target mRNAs. The expression and function of miRNAs have ...