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Tag Archives: Linear output

Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols

April 6, 2015 Leave a comment 4,655 Views

rna-seq

Recently, a number of protocols extending RNA-sequencing to the single-cell regime have been published. However, we were concerned that the additional steps to deal with such minute quantities of input sample would introduce serious biases that would make analysis of ...

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Recent RNA-SEQ Pubs

Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones
8 March 2021
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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