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Tag Archives: literature

Looking for Loners – A new algorithm opens doors for detecting rare cell types in mRNA sequencing

December 2, 2015 Leave a comment 2,603 Views

rna-seq

From The Scientist By Karen Zusi The Paper Individuality The advent of mRNA sequencing for individual cells has given scientists unprecedented insight into the diversity of cell populations once thought to be uniform. But finding an analysis sensitive enough to ...

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Recent RNA-SEQ Pubs

Transcriptomic analysis provides insights into candidate genes and molecular pathways involved in growth of Manila clam Ruditapes philippinarum
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Nuclear RNA purification by flow cytometry to study nuclear processes in plants
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Co-expression of glutamatergic and autism-related genes in the hippocampus of male mice with disturbances of social behavior
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

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    RNA sequencing brings hope for endangered wild cats

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