Tag Archives: long read sequencing

L-RAPiT: Long Read Analysis Pipeline for Transcriptomics

rna-seq

L-RAPiT: Long Read Analysis Pipeline for Transcriptomics is an easy-to-use publicly available pipeline which allows for analysis of long read RNA-sequencing data within a cloud environment; namely, Google Colaboratory. L-RAPiT is available at the following address: https://github.com/Theo-Nelson/long-read-sequencing-pipeline Citation: Nelson, T.M.; ...

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Template-switching artifacts resemble alternative polyadenylation

rna-seq

Alternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally disregarded, while alternative polyadenylation artifacts are attributed to internal priming. Researchers ...

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