Tag Archives: long read sequencing

L-RAPiT: Long Read Analysis Pipeline for Transcriptomics is an easy-to-use publicly available pipeline which allows for analysis of long read RNA-sequencing data within a cloud environment; namely, Google Colaboratory. L-RAPiT is available at the following address: https://github.com/Theo-Nelson/long-read-sequencing-pipeline Citation: Nelson, T.M.; ...

Many applications of multiple sequence alignments (MSA) involve working with sequences which are not ideal – they can be incomplete, contain errors or just be highly divergent with many mismatches. This is very common when working with high throughput sequencing ...

RNA sequencing using next-generation sequencing technologies (NGS) is currently the standard approach for gene expression profiling, particularly for large-scale high-throughput studies. NGS technologies comprise high throughput, cost efficient...

Structural variants (SVs) may be an underestimated cause of hereditary cancer syndromes given the current limitations of short-read next-generation sequencing. Here researchers from the University of...

Alternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally disregarded, while alternative polyadenylation artifacts are attributed to internal priming. Researchers ...

Significant Enhancements to Sequel System Enable >99% Accuracy for Single-Molecule Reads up to 15 kb; Throughput Increases by 2- to 4-Fold Pacific Biosciences of California, Inc. a provider of high-quality sequencing of genomes, transcriptomes and epigenomes, today announced major enhancements ...

In cancer cells, genetic errors wreak havoc. Misspelled genes, as well as structural variations—larger-scale rearrangements of DNA that can encompass large chunks of chromosomes—disturb carefully balanced mechanisms that have evolved to regulate cell growth. Genes that are normally silent are...