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Tag Archives: mapped reads

EMSAR – estimation of transcript abundance from RNA-seq data by mappability-based segmentation and reclustering

September 17, 2015 Leave a comment 4,413 Views

RNA-seq has been widely used for genome-wide expression profiling. RNA-seq data typically consists of tens of millions of short sequenced reads from different transcripts. However, due to sequence similarity among genes and among isoforms, the source of a given read ...

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Recent RNA-SEQ Pubs

Altered circular RNA expression profiles in an ovalbumin-induced murine model of allergic rhinitis
28 February 2021
Analysis of the transcriptomic, metabolomic, and gene regulatory responses to Puccinia sorghi in maize
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SPARC inhibition accelerates NAFLD-associated hepatocellular carcinoma development by dysregulating hepatic lipid metabolism
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PARP1-RNA interaction analysis: PARP1 regulates the expression of extracellular matrix-related genes in HK-2 renal proximal tubular epithelial cells
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Characterizing Escherichia coli's transcriptional response to different styrene exposure modes reveals novel toxicity and tolerance insights
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Bovine neonate is deficient in innate immunity at birth
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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    scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases

    11 hours ago
  • rna-seq

    DEBKS – a tool to detect differentially expressed circular RNA

    12 hours ago
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    Featured RNA-Seq Job – Staff Bioinformatics Scientist, RNA-Seq

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