When the human genome was first sequenced, experts predicted they would find about 100,000 genes. The actual number has turned out to be closer to 20,000, just a few thousand more than fruit flies have. The question logically arose: how ...
Read More »Scientists discover how disruptive genetic RNA is made
Israeli-led research finds circular RNA puts brakes on gene expression, could cause degenerative disease from Times of Israeli by Andrew Tobin An Israeli-led research team has discovered how a mysterious circular type of genetic molecule is produced, and that might ...
Read More »PLEK: a tool for predicting long non-coding RNAs and messenger RNAs based on an improved k-mer scheme
High-throughput transcriptome sequencing (RNA-seq) technology promises to discover novel protein-coding and non-coding transcripts, particularly the identification of long non-coding RNAs (lncRNAs) from de novo sequencing data. This requires tools that are not restricted by prior gene annotations, genomic sequences and ...
Read More »Genomic-scale exchange of mRNA between a parasitic plant and its hosts
Movement of RNAs between cells of a single plant is well documented, but cross-species RNA transfer is largely unexplored. Cuscuta pentagona (dodder) is a parasitic plant that forms symplastic connections with its hosts and takes up host messenger RNAs (mRNAs). ...
Read More »Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut ...
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