Although metagenomics and metatranscriptomics are commonly used to identify bacteria and viruses in human samples, few studies directly compare...
Read More »Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO
dsRNA is the genetic material of important viruses and a key component of RNA interference-based immunity in eukaryotes. Previous studies have noted difficulties in determining the sequence of dsRNA...
Read More »How to normalize metatranscriptomic count data for differential expression analysis
Differential expression analysis on the basis of RNA-Seq count data has become a standard tool in transcriptomics. Several studies have shown that prior normalization of the data is crucial for a reliable detection of transcriptional differences. Until now it has ...
Read More »UW-Madison lab decodes lake bacterial cycles with RNA sequencing
by Shelby Lyon of the Journal Sentinel – When a sheriff’s deputy found a van parked at the edge of Sparkling Lake in Vilas County at 2 a.m. on July 7, chances are he expected to find something nefarious afoot. ...
Read More »Ultrafast RNA-Seq Analysis Tool Could Change How Infectious Diseases Are Diagnosed
SALT LAKE CITY-Scientists at the University of Utah School of Medicine, ARUP Laboratories, and IDbyDNA, Inc., have developed ultra-fast, meta-genomics analysis software called Taxonomer that dramatically improves the accuracy and speed of pathogen detection. In a paper published today in ...
Read More »RNA-Seq Equally as Sensitive as 16S Metagenomics for Detection of Bacterial Pathogens
Rodents are major reservoirs of pathogens responsible for numerous zoonotic diseases in humans and livestock. Assessing their microbial diversity at both the individual and population level is crucial for monitoring endemic infections and revealing microbial association patterns within reservoirs. Recently, ...
Read More »The Consortium for Sequencing the Food Supply Chain
IBM Research and Mars tackle global health with food safety partnership The significant threat of food borne illness affects nearly every industry on every continent from government and healthcare to agriculture and retail, yet the massive issue continues to puzzle ...
Read More »Choosing a next-generation sequencing pipeline
The next-generation sequencing (NGS) technologies are revolutionary tools which have made possible achieving remarkable advances in genetics since the beginning of the twenty-first century. Thanks to the possibility to produce large amount of sequence data, these tools are going to ...
Read More »Next-generation sequencing technologies and their impact on microbial genomics
Next-generation sequencing technologies have had a dramatic impact in the field of genomic research through the provision of a low cost, high-throughput alternative to traditional capillary sequencers. These new sequencing methods have surpassed their original scope and now provide a ...
Read More »Analysis of Microbial Communities from 16S rRNA Sequencing
Dr. Christopher Taylor, Assistant Professor, Department of Computer Science, University of New Orleans.
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