Although metagenomics and metatranscriptomics are commonly used to identify bacteria and viruses in human samples, few studies directly compare...
Read More »On Demand Webinars – Maximize usable RNA-seq reads with an effective rRNA removal strategy
Efficient rRNA removal from mammalian, bacterial, plant and yeast samples for RNA-seq and metatranscriptomics studiesThe more effective your rRNA removal strategy, the greater your ability to achieve usable reads in RNA-seq. Not only does rRNA...
Read More »Customized workflow development and data modularization concepts for RNA-Sequencing experiments
RNA-Sequencing (RNA-Seq) has become a widely used approach to study quantitative and qualitative aspects of transcriptome data. The variety of RNA-Seq protocols, experimental study designs and the characteristic properties of the organisms under investigation greatly affect downstream and comparative analyses. ...
Read More »SAMSA – a comprehensive metatranscriptome analysis pipeline
Although metatranscriptomics-the study of diverse microbial population activity based on RNA-seq data-is rapidly growing in popularity, there are limited options for biologists to analyze this type of data. Current approaches for processing metatranscriptomes rely on restricted databases and a dedicated ...
Read More »A pipeline for rapid gene discovery and expression analysis of a plant host and its obligate parasite
Current and emerging plant diseases caused by obligate parasitic microbes such as rusts, downy mildews, and powdery mildews threaten worldwide crop production and food safety. These obligate parasites are typically unculturable in the laboratory, posing technical challenges to characterize them ...
Read More »UW-Madison lab decodes lake bacterial cycles with RNA sequencing
by Shelby Lyon of the Journal Sentinel – When a sheriff’s deputy found a van parked at the edge of Sparkling Lake in Vilas County at 2 a.m. on July 7, chances are he expected to find something nefarious afoot. ...
Read More »Comparison of library preparation methods reveals their impact on interpretation of metatranscriptomic data
Metatranscriptomics is rapidly expanding our knowledge of gene expression patterns and pathway dynamics in natural microbial communities. However, to cope with the challenges of environmental sampling, various rRNA removal and cDNA synthesis methods have been applied in published microbial metatranscriptomic ...
Read More »