RNA-Seq Blog Transcriptome Research & Industry News
RNA-seq is a reference technology for determining alternative splicing at genome-wide level. Exon arrays remain widely used for the analysis of gene...
Read More »A viable alternative to RNA-seq for detection of alternative splicing events?
EGSEA – easy and efficient ensemble gene set testing
Gene set enrichment analysis is a popular approach for prioritising the biological processes perturbed in genomic datasets. The Bioconductor project hosts over 80 software packages capable of gene set...
Read More »Global and targeted approaches to single-cell transcriptome characterization
Analysing transcriptomes of cell populations is a standard molecular biology approach to understand how cells function. Recent methodological development has allowed performing similar experiments on single cells. This has opened up the possibility to examine samples with limited cell number, ...
Read More »Researchers find that modern microarrays can outperform RNA-Seq in terms of reproducibility and cost
RNA sequencing (RNA-seq) and microarrays are two transcriptomics techniques aimed at the quantification of transcribed genes and their isoforms. Here researchers from the Luxembourg Institute of Health compare the latest Affymetrix HTA 2.0 microarray with Illumina 2000 RNA-seq for the ...
Read More »A probabilistic approach for automated discovery of perturbed genes using expression data from microarray or RNA-Seq
In complex diseases, alterations of multiple molecular and cellular components in response to perturbations are indicative of disease physiology. While expression level of genes from high-throughput analysis can vary among patients, the common path among disease progression suggests that the ...
Read More »Study suggests that microarrays can perform on nearly equal footing with RNA-Seq
The arrival of RNA-seq as a high-throughput method competitive to the established microarray technologies has necessarily driven a need for comparative evaluation. To date, cross-platform comparisons of these technologies have been relatively few in number of platforms analyzed and were ...
Read More »Differences in intensity-dependence between RNA-seq and microarrays
Understanding the differences between microarray and RNA-Seq technologies for measuring gene expression is necessary for informed design of experiments and choice of data analysis methods. Previous comparisons have come to sometimes contradictory conclusions. Reseashers from Princeton University suggest that this ...
Read More »Comparison of atopic dermatitis transcriptome profiling by microarray and RNA-Seq
Genomic profiling of lesional and nonlesional skin of patients with atopic dermatitis (AD) using microarrays has led to increased understanding of AD and identification of novel therapeutic targets. However, the limitations of microarrays might decrease detection of AD genes. These ...
Read More »Dual RNA-Seq enables computational prediction of molecular pathogen-host interactions
Inference of inter-species gene regulatory networks based on gene expression data is an important computational method to predict pathogen-host interactions (PHIs). Both the experimental setup and the nature of PHIs exhibit certain characteristics. First, besides an environmental change, the battle ...
Read More »Microarray Not Fade Away
Yes, many are attracted to next-generation sequencing, but loyalty to chip-based phenotyping has its rewards, particularly in screens of large sample sets. from Genetic Engineering News – by Lisa Heiden The microarray, the legacy technology for measuring gene expression, will ...
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