The ability to simultaneously characterize the bacterial and host expression programs during infection would facilitate a comprehensive understanding of pathogen-host interactions. Although RNA sequencing (RNA-seq) has greatly advanced our ability to study the transcriptomes of prokaryotes and eukaryotes separately, limitations ...
Read More »Experimental Design Considerations for Microbial RNA-seq
RNA-seq is being used increasingly for gene expression studies and it is revolutionizing the fields of genomics and transcriptomics. However, the field of RNA-seq analysis is still evolving. Now, researchers at the University of Tennessee, Knoxville specifically designed a study ...
Read More »The Consortium for Sequencing the Food Supply Chain
IBM Research and Mars tackle global health with food safety partnership The significant threat of food borne illness affects nearly every industry on every continent from government and healthcare to agriculture and retail, yet the massive issue continues to puzzle ...
Read More »CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies
In ecological studies microbial diversity is nowadays mostly assessed via the detection of phylogenetic marker genes such as 16S ribosomal RNA. However, PCR amplification of these marker genes produces a significant amount of artificial sequences often referred to as chimeras. ...
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