"Hidden" associations between the human virome and human health and diseaseHuman-resident microbes can influence both health and disease. Investigating the microbiome using next-generation sequencing(*1) technology has revealed examples...
Read More »Study provides first look at sperm microbiome using RNA sequencing sensitive enough to detect bacteria
A new collaborative study published by a research team from the Wayne State University School of Medicine, the CReATe Fertility Centre and the University of Massachusetts Amherst provides the first in-depth look at the microbiome of human...
Read More »A crash course in sequencing for a microbiologist
For the last 40 years, “Sanger sequencing” allowed to unveil crucial secrets of life. However, this method of sequencing has been time-consuming, laborious and remains expensive even today. Human...
Read More »A fast and robust protocol for metataxonomic analysis using RNA-Seq data
Metagenomics is a rapidly emerging field aimed to analyze microbial diversity and dynamics by studying the genomic content of the microbiota. Metataxonomics tools analyze high-throughput sequencing data, primarily from 16S rRNA gene sequencing and DNAseq, to identify microorganisms and viruses ...
Read More »SAMSA – a comprehensive metatranscriptome analysis pipeline
Although metatranscriptomics-the study of diverse microbial population activity based on RNA-seq data-is rapidly growing in popularity, there are limited options for biologists to analyze this type of data. Current approaches for processing metatranscriptomes rely on restricted databases and a dedicated ...
Read More »Total RNA Sequencing reveals microbial communities in human blood and disease specific effects
An increasing body of evidence suggests an important role of the human microbiome in health and disease. Researchers at UCLA have developed a ‘lost and found’ pipeline, which examines high quality unmapped sequence reads for microbial taxonomic classification. Using this ...
Read More »Featured RNA-Seq Job – Informatics Institute Next-Generation Sequencing (NGS) Analyst/Consultant
University of Minnesota, Twin Cities Duties/Responsibilities: The University of Minnesota Informatics Institute (UMII) and University of Minnesota Genomics Center (UMGC) seek a skilled, Ph.D.-level analyst to serve as both internal NGS analyst and client-facing NGS consultant. The UMGC is the ...
Read More »ALDEx2 – characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis
Experimental designs that take advantage of high-throughput sequencing to generate datasets include RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), sequencing of 16S rRNA gene fragments, metagenomic analysis and selective growth experiments. In each case the underlying data are similar and ...
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