Tag Archives: microfluidics

Optically decodable beads link the identity of a sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and ...

Kyoto University researchers report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA...

Single-cell RNA-seq reveals the cellular heterogeneity inherent in the population of cells, which is very important in many clinical and research applications. Recent advances in droplet microfluidics have achieved the automatic isolation, lysis...

1CellBiO Inc., a leading single-cell analysis company serving the biomedical research community, today announced that it has signed a reseller and license agreement with Hangzhou Chengyuan Genomics Company (Chengyuan), a premier life-science research and development start-up based in Hangzhou, China. Under the ...

DroNc-Seq — a technology that merges single-nucleus RNA sequencing with microfluidics — brings new scale to gene expression studies in complex tissues. from The Broad Institute by Tom Ulrich –  Last year Broad researchers described a single-nucleus RNA sequencing method called sNuc-Seq. This ...

Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for capturing the unbiased diversity of cells. Droplet microfluidics is among the most promising candidates for ...

Prevalent single cell RNA amplification and sequencing chemistries mainly focus on polyadenylated RNAs in eukaryotic cells by using oligo(dT) primers for reverse transcription. Researchers from Peking University have developed a new RNA amplification method, “easier-seq”, to reverse transcribe and amplify ...

The study of single cells has evolved over the past several years to include expression and genomic analysis of an increasing number of single cells. Several studies have demonstrated wide spread variation and heterogeneity within cell populations of similar phenotype. ...

Ioannis (Jiannis) Ragoussis – Microfluidic Single Cell Exome seq and RNA seq analysis of Tumor Composition Human breast tumors have been shown to exhibit extensive inter- and intra-tumor heterogeneity. While recent advances in genomic technologies have allowed us to deconvolute ...

Rare cell subtypes can profoundly impact the course of human health and disease, yet their presence within a sample is often missed with bulk molecular analysis. Single-cell analysis tools such as FACS, FISH-FC and single-cell barcode-based sequencing can investigate cellular ...