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Tag Archives: miRge

miRge – A Multiplexed Method of Processing Small RNA-Seq Data to Determine MicroRNA Entropy

November 19, 2015 Leave a comment 5,059 Views

rna-seq

Small RNA RNA-seq for microRNAs (miRNAs) is a rapidly developing field where opportunities still exist to create better bioinformatics tools to process these large datasets and generate new, useful analyses. Researchers at Johns Hopkins University School of Medicine have built ...

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Recent RNA-SEQ Pubs

Melatonin promotes potassium deficiency tolerance by regulating HAK1 transporter and its upstream transcription factor NAC71 in wheat
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Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic <em>orco</em> mutation in honey bees
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RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes
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Transcriptomic data showing differentially expressed genes between Notch3 and Notch4 deleted mice
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Comparative transcriptome analysis of roots, stems, and leaves of <em>Pueraria lobata</em> (Willd.) Ohwi: identification of genes involved in isoflavonoid biosynthesis
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The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    Learn more about available bioinformatics tools and pipelines to analyze RNA sequencing data

    1 day ago
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    New search engine for single cell atlases

    1 day ago
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    Yale researchers identify tumor reactive immune cells with RNA sequencing to help fight against advanced melanoma

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    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    2 days ago
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    RNA sequencing brings hope for endangered wild cats

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    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

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    Uncovering the Anti-Myeloma Resistance Files

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