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Tag Archives: miRNA quantification

Comparison of reproducibility, accuracy, sensitivity, and specificity of miRNA quantification platforms

December 20, 2019 Leave a comment 1,875 Views

rna-seq

Given the increasing interest in their use as disease biomarkers, the establishment of reproducible, accurate, sensitive, and specific platforms for...

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Recent RNA-SEQ Pubs

Molecular characterization and analysis of the ATPase ASNA1 homolog gene of Eimeria tenella in a drug sensitive strain and drug resistant strains
27 February 2021
Hydrogen Gas Therapy Attenuates Inflammatory Pathway Signaling in Septic Mice
27 February 2021
Genome-wide investigation and expression analysis of MACPF gene family reveals its immune role in response to bacterial challenge of Manila clam
27 February 2021
Response of the ileum transcriptome to probiotic and fructo-oligosaccharides in Taiping chicken
27 February 2021
Transcriptome Profiling of Long Non-coding RNAs During the Atlantic Salmon Smoltification Process
27 February 2021
Genome-wide identification and functional characterization of natural antisense transcripts in Salvia miltiorrhiza
27 February 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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  • rna-seq

    scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases

    53 mins ago
  • rna-seq

    DEBKS – a tool to detect differentially expressed circular RNA

    2 hours ago
  • rna-seq

    Single-cell RNA and protein expression in relation to cell cycle progression

    3 days ago
  • rna-seq

    Evaluating capture sequence performance for single-cell CRISPR activation experiments

    3 days ago
  • rna-seq

    Seven Bridges Selected by Pfizer to Support Data Management Solution for Single-Cell RNA Sequencing Data

    3 days ago
  • rna-seq

    Single-cell mapper (scMappR) – using scRNA-seq to infer the cell-type specificities of differentially expressed genes

    4 days ago
  • rna-seq

    Featured RNA-Seq Job – Staff Bioinformatics Scientist, RNA-Seq

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