RNA-Seq Blog Transcriptome Research & Industry News
Many tools have been developed to profile microRNA (miRNA) expression from small RNA-seq data. These tools must contend with several issues: the small...
Read More »miR-MaGiC improves quantification accuracy for small RNA-seq
Small RNA and degradome sequencing reveals the roles of microRNAs in seed expansion in peanut
Seed expansion in peanut is a complex biological process involving many gene regulatory pathways. MicroRNAs (miRNAs) play important regulatory roles in plant growth and development, but little is known...
Read More »An evaluation of bioinformatics approaches for next-generation sequencing analysis of microRNAs
MicroRNAs (miRNAs) are key post-transcriptional regulators that affect protein translation by targeting mRNAs. Their role in disease etiology and toxicity are well recognized. Given the rapid advancement of next-generation sequencing techniques, miRNA profiling has been increasingly conducted with RNA-seq, namely ...
Read More »unitas – the universal tool for annotation of small RNAs
Next generation sequencing is a key technique in small RNA biology research that has led to the discovery of functionally different classes of small non-coding RNAs in the past years. However, reliable annotation of the extensive amounts of small non-coding ...
Read More »Small RNA Library Construction for Exosomal RNA Sequencing on the Ion Torrent PGM™ and Ion S5™ System
Next-generation deep sequencing (NGS) technology represents a powerful and innovative approach to profile small RNA. Currently, there are a number of large-scale and benchtop sequencing platforms available on the market. Although each platform is relatively straightforward to operate, constructing cDNA ...
Read More »Small-RNA Sequencing of Single Cells
The ability to profile mRNAs in single cells has transformed the molecular identification and the characterization of cell types, states and rare cellular phenotypes. However, current single-cell methods are restricted to long RNAs, primarily mRNAs. Several classes of small RNAs ...
Read More »Post Doc position – Computational biology and Experimental biology – miRNAs and non-coding RNAs lab
Added on: 15/09/2016 – Expires on: 15/10/2016 We are seeking a postdoctoral candidate to join the “microRNAs and non-coding RNAs in development and disease” team led by Dr. Francesco Nicassio. The Center for Genomic Science of IIT@SEMM (https://www.iit.it/it/centri/cgs-semm), located in ...
Read More »MULSEA – Mutual enrichment in aggregated ranked lists with applications to gene expression regulation
It is often the case in biological measurement data that results are given as a ranked list of quantities-for example, differential expression (DE) of genes as inferred from microarrays or RNA-seq. Recent years brought considerable progress in statistical tools for ...
Read More »A workflow for analysis of small RNA sequencing data
Small RNAs (size 20-30 nt) of various types have been actively investigated in recent years, and their subcellular compartmentalization and relative concentrations are likely to be of importance to their cellular and physiological functions. Comprehensive data on this subset of ...
Read More »gsa4mirna – Integrated gene set analysis for microRNA studies
Functional interpretation of miRNA expression data is currently done in a three step procedure: select differentially expressed miRNAs, find their target genes, and carry out gene set overrepresentation analysis Nevertheless, major limitations of this approach have already been described at ...
Read More »