microRNAs (miRNAs) are small non-coding RNAs (~22 nts) that are considered central post-transcriptional regulators of gene expression and key components in many pathological conditions. Next-Generation Sequencing (NGS) technologies have led to inexpensive, massive data production, revolutionizing every research aspect in ...
Read More »BrumiR – a toolkit for de novo discovery of microRNAs from sRNA-seq data
MicroRNAs (miRNAs) are small non-coding RNAs that are key players in the regulation of gene expression. In the last decade, with the increasing accessibility of high-throughput sequencing technologies, different methods have been developed to identify miRNAs...
Read More »seqclusterViz – visualization of the small RNA transcriptome
The study of small RNAs provides us with a deeper understanding of the complexity of gene regulation within cells. Of the different types of small RNAs, the most important in mammals are miRNA, tRNA fragments and piRNAs. Using small RNA-seq ...
Read More »RNA-Seq reveals the molecular basis for the single-nucleotide precision of primary microRNA processing
Microprocessor, composed of DROSHA and its cofactor DGCR8, initiates microRNA (miRNA) biogenesis by processing the primary transcripts of miRNA (pri-miRNAs). Researchers at the Seoul...
Read More »RNA-Seq reveals large differences in small RNA composition between human biofluids
Extracellular microRNAs (miRNAs) and other small RNAs are implicated in cellular communication and may be useful as disease biomarkers. UCSF researchers systematically compared small RNAs in 12 human biofluid types using RNA sequencing...
Read More »Crohn’s Disease subtype accurately predicted through miRNA sequencing
Crohn’s disease (CD), a chronic inflammatory condition of the intestinal tract, has emerged as a global disease, with rates steadily increasing over...
Read More »miR-MaGiC improves quantification accuracy for small RNA-seq
Many tools have been developed to profile microRNA (miRNA) expression from small RNA-seq data. These tools must contend with several issues: the small...
Read More »Small RNA and degradome sequencing reveals the roles of microRNAs in seed expansion in peanut
Seed expansion in peanut is a complex biological process involving many gene regulatory pathways. MicroRNAs (miRNAs) play important regulatory roles in plant growth and development, but little is known...
Read More »An evaluation of bioinformatics approaches for next-generation sequencing analysis of microRNAs
MicroRNAs (miRNAs) are key post-transcriptional regulators that affect protein translation by targeting mRNAs. Their role in disease etiology and toxicity are well recognized. Given the rapid advancement of next-generation sequencing techniques, miRNA profiling has been increasingly conducted with RNA-seq, namely ...
Read More »unitas – the universal tool for annotation of small RNAs
Next generation sequencing is a key technique in small RNA biology research that has led to the discovery of functionally different classes of small non-coding RNAs in the past years. However, reliable annotation of the extensive amounts of small non-coding ...
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