Tag Archives: mirna

Seed expansion in peanut is a complex biological process involving many gene regulatory pathways. MicroRNAs (miRNAs) play important regulatory roles in plant growth and development, but little is known...

MicroRNAs (miRNAs) are key post-transcriptional regulators that affect protein translation by targeting mRNAs. Their role in disease etiology and toxicity are well recognized. Given the rapid advancement of next-generation sequencing techniques, miRNA profiling has been increasingly conducted with RNA-seq, namely ...

Next generation sequencing is a key technique in small RNA biology research that has led to the discovery of functionally different classes of small non-coding RNAs in the past years. However, reliable annotation of the extensive amounts of small non-coding ...

Next-generation deep sequencing (NGS) technology represents a powerful and innovative approach to profile small RNA. Currently, there are a number of large-scale and benchtop sequencing platforms available on the market. Although each platform is relatively straightforward to operate, constructing cDNA ...

The ability to profile mRNAs in single cells has transformed the molecular identification and the characterization of cell types, states and rare cellular phenotypes. However, current single-cell methods are restricted to long RNAs, primarily mRNAs. Several classes of small RNAs ...

Added on: 15/09/2016 – Expires on: 15/10/2016 We are seeking a postdoctoral candidate to join the “microRNAs and non-coding RNAs in development and disease” team led by Dr. Francesco Nicassio. The Center for Genomic Science of [email protected] (https://www.iit.it/it/centri/cgs-semm), located in ...

It is often the case in biological measurement data that results are given as a ranked list of quantities-for example, differential expression (DE) of genes as inferred from microarrays or RNA-seq. Recent years brought considerable progress in statistical tools for ...

Small RNAs (size 20-30 nt) of various types have been actively investigated in recent years, and their subcellular compartmentalization and relative concentrations are likely to be of importance to their cellular and physiological functions. Comprehensive data on this subset of ...

Functional interpretation of miRNA expression data is currently done in a three step procedure: select differentially expressed miRNAs, find their target genes, and carry out gene set overrepresentation analysis Nevertheless, major limitations of this approach have already been described at ...

Performing qPCR-based profiling of microRNA from single cells or small cell numbers? Our July 12 webinar will definitely help you get the most out of your precious samples! The webinar focuses on a new, streamlined process for differential expression profiling based on ...