Tag Archives: mirna

The ability to profile mRNAs in single cells has transformed the molecular identification and the characterization of cell types, states and rare cellular phenotypes. However, current single-cell methods are restricted to long RNAs, primarily mRNAs. Several classes of small RNAs ...

Added on: 15/09/2016 – Expires on: 15/10/2016 We are seeking a postdoctoral candidate to join the “microRNAs and non-coding RNAs in development and disease” team led by Dr. Francesco Nicassio. The Center for Genomic Science of IIT@SEMM (https://www.iit.it/it/centri/cgs-semm), located in ...

It is often the case in biological measurement data that results are given as a ranked list of quantities-for example, differential expression (DE) of genes as inferred from microarrays or RNA-seq. Recent years brought considerable progress in statistical tools for ...

Small RNAs (size 20-30 nt) of various types have been actively investigated in recent years, and their subcellular compartmentalization and relative concentrations are likely to be of importance to their cellular and physiological functions. Comprehensive data on this subset of ...

Functional interpretation of miRNA expression data is currently done in a three step procedure: select differentially expressed miRNAs, find their target genes, and carry out gene set overrepresentation analysis Nevertheless, major limitations of this approach have already been described at ...

Performing qPCR-based profiling of microRNA from single cells or small cell numbers? Our July 12 webinar will definitely help you get the most out of your precious samples! The webinar focuses on a new, streamlined process for differential expression profiling based on ...

Circulating biomarkers are urgently needed in HCC. miRNA are noncoding RNA that regulate mRNA expression and are detectable in tumor tissue and extracellular compartments. This pilot study explored the feasibility of using next-generation RNA sequencing to characterize miRNA abundance in ...

Small RNA-Seq has emerged as a powerful tool in transcriptomics, gene expression profiling and biomarker discovery. Sequencing cell-free nucleic acids, particularly microRNA (miRNA), from liquid biopsies additionally provides exciting possibilities for molecular diagnostics, and might help establish disease-specific biomarker signatures. ...

Various types of mutation and editing (M/E) events in microRNAs (miRNAs) can change the stabilities of pre-miRNAs and/or complementarities between miRNAs and their targets. Small RNA (sRNA) high-throughput sequencing (HTS) profiles can contain many mutated and edited miRNAs. Systematic detection ...

Large-scale sequencing experiments are complex and require a wide spectrum of computational tools to extract and interpret relevant biological information. This is especially true in projects where individual processing and integrated analysis of both small RNA and complementary RNA data ...