Tag Archives: Mix2

Accuracy of transcript quantification with RNA-Seq is negatively affected by positional fragment bias. Researchers at Lexogen GmbH introduce Mix2 (rd. “mixquare”), a transcript quantification method which uses a mixture of probability distributions to model and thereby neutralize the effects of positional ...

Mix2 (rd. “mixquare”) yields highly accurate concentration estimates for gene isoforms by adapting to the positional coverage bias in RNA-Seq data. This leads to higher accuracy in the detection of differential expression of genes and gene isoforms. Mix2 enables repeatable ...

Quantification of RNA transcripts with RNA-Seq is inaccurate due to positional fragmentation bias, which is not represented appropriately by current statistical models of RNA-Seq data. Another, less investigated, source of error is the inaccuracy of transcript start and end annotations. ...