Tag Archives: molecular barcoding

Formalin-fixed paraffin-embedded (FFPE) tissues are the most abundant archivable specimens in clinical tissue banks, but unfortunately incompatible with single-cell level transcriptome sequencing due to RNA...

RNA-Seq measures gene expression by counting sequence reads belonging to unique cDNA fragments. Molecular barcodes commonly in the form of random nucleotides were recently introduced to improve gene expression measures by detecting amplification duplicates, but are susceptible to errors generated ...

The development of single-cell RNA-seq has allowed the detection of gene expression at an anatomical resolution that is not accessible by bulk RNA-seq approaches. While single-cell RNA-seq methods have been successfully used to identify new cell types in complex tissues, ...