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Tag Archives: molecular counts

dropEst – pipeline for accurate estimation of molecular counts in droplet-based single-cell RNA-seq experiments

July 16, 2018 Leave a comment 2,367 Views

rna-seq

Recent single-cell RNA-seq protocols based on droplet microfluidics use massively multiplexed barcoding to enable simultaneous measurements of transcriptomes for thousands of individual cells. The increasing complexity of such data creates challenges for subsequent computational processing...

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Recent RNA-SEQ Pubs

Development and Multi-Data Set Verification of an RNA Binding Protein Signature for Prognosis Prediction in Glioma
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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