Tag Archives: mouse

Recent transcriptional profiling technologies are uncovering previously-undefined cell populations and molecular markers at an unprecedented pace. While single cell RNA (scRNA) sequencing is an attractive...

Metastasis-initiating cells dynamically adapt to the distinct microenvironments of different organs, but these early adaptations are poorly understood due to the limited sensitivity of in situ transcriptomics...

The RNA sequencing approach has been broadly used to provide gene-, pathway-, and network-centric analyses for various cell and tissue samples. However, thus far, rich cellular information carried in tissue samples has not been thoroughly characterized from RNA-Seq data. Therefore, ...

Targeted RNA sequencing (CaptureSeq) uses oligonucleotide probes to capture RNAs for sequencing, providing enriched read coverage, accurate measurement of gene expression, and quantitative expression data. Researchers from the European Bioinformatics Institute applied CaptureSeq to refine transcript annotations in the current ...

Clarifying gene expression in narrowly defined neuronal populations can provide insight into cellular identity, computation, and functionality. Here, researchers at the Howard Hughes Medical Institute used next-generation RNA sequencing (RNA-seq) to produce a quantitative, whole genome characterization of gene expression ...

One of the major properties of genes is their expression pattern. Notably, genes are often classified as tissue specific or housekeeping. This property is of interest to molecular evolution as an explanatory factor of, e.g. evolutionary rate, as well as ...

from Bio-IT World By Aaron Krol An adult mouse’s brain, an object not much bigger than the last joint of your pinky finger, contains around 75 million neurons. At the Allen Institute for Brain Science in Seattle, the Mouse Cell ...

Two new studies in mice demonstrate how a father’s diet affects levels of specific small RNAs in his sperm, which in turn can affect gene regulation in offspring. These results add to the growing list of ways in which a ...

Gene targeting is a protocol for introducing a mutation to a specific gene in an organism. Because of the importance of in vivo assessment of gene function and modeling of human diseases, this technique has been widely adopted to generate ...