Recommendations for utilizing targeted RNA sequencing to evaluate potential spliceogenic variants

May 22, 2018 Leave a comment 2,277 Views

PCR-based RNA splicing assays are commonly used in diagnostic and research settings to assess the potential effects of variants of uncertain clinical significance in BRCA1 and BRCA2. The Evidence-based...

NMFP – a non-negative matrix factorization based preselection method to identify mRNA isoforms from RNA-seq data

February 4, 2016 Leave a comment 3,313 Views

The advent of next-generation RNA sequencing (RNA-seq) has greatly advanced transcriptomic studies, including system-wide identification and quantification of mRNA isoforms under various biological conditions. A number of computational methods have been developed to systematically identify mRNA isoforms in a high-throughput ...

Quantitative visualization of alternative exon expression from RNA-Seq data

January 30, 2015 Leave a comment 7,216 Views

Analysis of RNA sequencing (RNA-Seq) data revealed that the vast majority of human genes express multiple mRNA isoforms, produced by alternative pre-mRNA splicing and other mechanisms, and that most alternative isoforms vary in expression between human tissues (Pan et al. ...

PostDoc Position Available – Biostatistician

June 9, 2014 1 Comment 4,738 Views

Postdoctoral Biostatistician (High-throughput Genomics / Transcriptomics) Salary Range – £37491 to £40600 per annum Location – The Babraham Institute, Cambridge, United Kingdom Description – The Babraham Institute is a charity which is world renowned for its research in life sciences to ...

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Tag Archives: mRNA isoforms

Recommendations for utilizing targeted RNA sequencing to evaluate potential spliceogenic variants

NMFP – a non-negative matrix factorization based preselection method to identify mRNA isoforms from RNA-seq data

Quantitative visualization of alternative exon expression from RNA-Seq data

PostDoc Position Available – Biostatistician

RPKM, FPKM and TPM, clearly explained

How to Analyze RNA-Seq Data?

Visualization of nucleotide substitutions in the (micro)transcriptome

Comparison of TMM (edgeR), RLE (DESeq2), and MRN Normalization Methods

Life Technologies Releases New Research Tool: Oncomine NGS RNA-Seq Gene Expression Browser

DestVI – identification of continuums of cell types in spatial transcriptomics data

TAS-Seq – terminator-assisted solid-phase cDNA amplification and sequencing for scRNA-seq

Post-doc position available – single-cell multi-omics analyses in a first-in-human gene therapy trial

Single-cell map of heart failure suggests possible therapeutic targets

RNA sequencing reveals changed gene expression after heart surgery extends cardiomyocyte regeneration

DestVI – identification of continuums of cell types in spatial transcriptomics data

TAS-Seq – terminator-assisted solid-phase cDNA amplification and sequencing for scRNA-seq

Post-doc position available – single-cell multi-omics analyses in a first-in-human gene therapy trial

Single-cell map of heart failure suggests possible therapeutic targets

RNA sequencing reveals changed gene expression after heart surgery extends cardiomyocyte regeneration

RNA sequencing methods developer given career award

Amphioxus sequencing gives insight on vertebrate evolution