Developmental Analysis of Spliceosomal snRNA Isoform Expression with RNA-Seq

November 26, 2014 Leave a comment 4,169 Views

Pre-mRNA splicing is a critical step in eukaryotic gene expression that contributes to proteomic, cellular and developmental complexity. Small nuclear (sn)RNAs are core spliceosomal components, however, the extent to which differential expression of snRNA isoforms regulates splicing is completely unknown. ...

SUPPA – a super-fast pipeline for alternative splicing analysis from RNA-Seq

September 9, 2014 Comments Off on SUPPA – a super-fast pipeline for alternative splicing analysis from RNA-Seq 6,865 Views

High-throughput RNA sequencing allows genome-wide analyses of pre-mRNA splicing across multiple conditions. However, the increasing number of available datasets represents a major challenge in terms of time and storage required for analyses. Now, a team led by researchers at the ...

RNA-Seq Blog Transcriptome Research & Industry News

Tag Archives: mRNA splicing

Developmental Analysis of Spliceosomal snRNA Isoform Expression with RNA-Seq

SUPPA – a super-fast pipeline for alternative splicing analysis from RNA-Seq

RNA-Seq data suggests that exitron splicing represents an additional cancer-driving mechanism beyond genetic alterations

SPEAQeasy – a scalable pipeline for expression analysis and quantification for R/bioconductor-powered RNA-seq analyses

ac4C-seq – quantitative nucleotide resolution profiling of RNA cytidine acetylation

Trips-Viz: an environment for the analysis of public and user-generated ribosome profiling data

IsoSeek – unbiased and UMI-informed sequencing of cell-free miRNAs at single-nucleotide resolution

Cell-level metadata are indispensable for documenting single-cell RNA sequencing datasets

Strategies for detecting and identifying biological signals amidst the variation commonly found in RNA sequencing data