Time: Thursday February 4, 2021, 10.00 GMT / 11.00 CET Description: RNA-Seq (RNA-sequencing or transcriptome sequencing) examines the quantity (quantification) and sequences (isoform identification) of RNA using various sequencing technologies. In this webinar, our technical expert will guide you through ...
Read More »Upcoming Webinar – Comprehensive Data Analysis of Eukaryotic mRNA-Seq
Time: Wednesday, December 9th 10:30 am-11:30 am PST| 1:30 pm-2:30 pm EST Speaker: Mario S. Izidoro Jr., Ph.D., Technical Support Scientist, Novogene Corporation Inc. Mario Izidoro has a pharmacy degree and a master’s degree in biochemistry and human physiology. During his ...
Read More »How to Analyze Single Cell RNA-Seq Data: Point, Click, Done
How to Analyze Single Cell RNA-Seq Data: Point, Click, Done June 24, 2020 11:00 a.m. EDT ************** *REGISTER* ************** Single cell mRNA sequencing allows for the identification of different cell subtypes in a progenitor population. During pancreatic development, Neurog3 positive ...
Read More »Differential gene expression analysis tools exhibit substandard performance for long non-coding RNA-sequencing data
Long non-coding RNAs (lncRNAs) are typically expressed at low levels and are inherently highly variable. This is a fundamental challenge for differential expression (DE) analysis. In this study, Ghent...
Read More »Recommendations for utilizing targeted RNA sequencing to evaluate potential spliceogenic variants
PCR-based RNA splicing assays are commonly used in diagnostic and research settings to assess the potential effects of variants of uncertain clinical significance in BRCA1 and BRCA2. The Evidence-based...
Read More »Optimized methodology for the generation of RNA-sequencing libraries from low-input starting material
RNA sequencing (RNA-seq) has become an important tool for examining the role of the transcriptome to biological processes. While RNA-seq has been widely adopted as a popular approach in many experimental designs, from gene discovery to mechanistic validation of targets, ...
Read More »Exercise encourages the cell to make more RNA copies of mitochondrial genes
Researchers have long suspected that the benefits of exercise extend down to the cellular level, but know relatively little about which exercises help cells rebuild key organelles that deteriorate with aging. A study published March 7 in Cell Metabolism found that exercise ...
Read More »Total RNA-seq reveals pharmacological effects on specific stages of mRNA synthesis
Pharmacological perturbation is a powerful tool for understanding mRNA synthesis, but identification of the specific steps of this multi-step process that are targeted by small molecules remains challenging. Here researchers from Harvard Medical School applied strand-specific total RNA sequencing (RNA-seq) ...
Read More »Power analysis at the isoform level
RNA-Sequencing (RNA-Seq) has become a routine technology for investigating gene expression differences in comparative transcriptomic studies. Differential expression (DE) analysis of the isoforms of genes is just emerging now that expression (read counts) can be estimated with higher accuracy at ...
Read More »miARma-Seq – a comprehensive tool for miRNA, mRNA and circRNA analysis
Large-scale RNAseq has substantially changed the transcriptomics field, as it enables an unprecedented amount of high resolution data to be acquired. However, the analysis of these data still poses a challenge to the research community. Many tools have been developed ...
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