Population scale sweeps of viral pathogens, such as SARS-CoV-2, that incorporate large numbers of asymptomatic or mild symptom patients present unique challenges for public health agencies trying to manage both travel and local spread. Physical...
Read More »Sparse full length sequencing – a highly multiplexed ribosomal RNA depletion-based RNA sequencing platform
The need to reduce per sample cost of RNA-seq profiling for scalable data generation has led to the emergence of highly multiplexed RNA-seq. These technologies utilize barcoding of cDNA sequences in order to combine multiple samples into a single sequencing ...
Read More »SPLiT-seq – single-cell profiling with split-pool barcoding
To facilitate scalable profiling of single cells, engineers at the University of Washington have developed split-pool ligation-based transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that labels the cellular origin of RNA through combinatorial barcoding. SPLiT-seq is compatible with fixed ...
Read More »A robust and cost-effective approach to sequence and analyze complete genomes of small RNA viruses
Next-generation sequencing (NGS) allows ultra-deep sequencing of nucleic acids. The use of sequence-independent amplification of viral nucleic acids without utilization of target-specific primers provides advantages over traditional sequencing methods and allows detection of unsuspected variants and co-infecting agents. However, NGS ...
Read More »Je – a suite of tools that accommodates complex barcoding strategies including unique molecular identifiers (UMIs)
The yield obtained from next generation sequencers has increased almost exponentially in recent years, making sample multiplexing common practice. While barcodes (known sequences of fixed length) primarily encode the sample identity of sequenced DNA fragments, barcodes made of random sequences ...
Read More »QuantSeq 3’ mRNA sequencing for RNA quantification
With the rapid development of NGS technologies, RNA-seq has become the new standard for transcriptome analysis. Although the price per base has been substantially reduced, sample preparation, sequencing and data processing are major cost factors in high throughput screenings. QuantSeq ...
Read More »The trade-off between increased multiplexing and decreasing sequencing depth in smallRNA-Seq
Small RNA sequencing can be used to gain an unprecedented amount of detail into the microRNA transcriptome. The relatively high cost and low throughput of sequencing bases technologies can potentially be offset by the use of multiplexing. However, multiplexing involves ...
Read More »PNAS Publication Demonstrates Power of Molecular Indexing™ Technology for Tracking RNA Molecules in RNA-Seq Library Preparation
PALO ALTO, Calif., Feb. 6, 2014 /PRNewswire/ — Cellular Research, Inc., today announced that its Molecular Indexing™ technology enables the absolute quantification of the starting copy number of an expressed gene, or RNA transcript, in an RNA sequencing (RNA-Seq) library. Data ...
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