Tag Archives: Mutations

Children’s Hospital Los Angeles Announces Development of Unique Targeted DNA and RNA Sequencing Panel Designed for Pediatric Cancer Research

rna-seq

LOS ANGELES–(BUSINESS WIRE)–Children’s Hospital Los Angeles (CHLA) and Thermo Fisher Scientific have agreed to develop a next-generation sequencing (NGS)-based panel designed specifically for pediatric cancer research. The assay would be CHLA’s first NGS panel designed to target biomarkers associated with ...

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Kleat – cleavage site analysis of transcriptomes

rna-seq

In eukaryotic cells, alternative cleavage of 3′ untranslated regions (UTRs) can affect transcript stability, transport and translation. For polyadenylated (poly(A)) transcripts, cleavage sites can be characterized with short-read sequencing using specialized library construction methods. However, for large-scale cohort studies as ...

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Gene-Editing Guide

rna-seq

from Harvard Univeristy by By SUE McGREEVEY New method identifies genome-wide off-target effects of CRISPR-Cas Harvard Medical School investigators at Massachusetts General Hospital have developed a method for detecting unwanted DNA breaks—across the entire genome of human cells—induced by the ...

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The statistical universe is a single patient – pathway analysis from “N-of-1” RNA-Seq data

rna-seq

The emergence of precision medicine allowed the incorporation of individual molecular data into patient care. Indeed, DNA sequencing predicts somatic mutations in individual patients. However, these genetic features overlook dynamic epigenetic and phenotypic response to therapy. Meanwhile, accurate personal transcriptome ...

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OncoRep: An n-of-1 reporting tool to support genome-guided treatment for breast cancer patients using RNA-sequencing

rna-seq

Breast cancer comprises multiple tumor entities associated with different biological features and clinical behaviors, making individualized medicine a powerful tool to bring the right drug to the right patient. Next generation sequencing of RNA (RNA-Seq) is a suitable method to ...

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