Tag Archives: nanopore

Current methods for determining RNA structure with short-read sequencing cannot capture most differences between distinct transcript isoforms. Researchers at A*STAR, Singapore present RNA structure analysis using nanopore sequencing...

The number of studies using third-generation sequencing utilizing Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) is rapidly increasing in many different research areas. Among them, plant full-length single-molecule transcriptome studies have mostly used PacBio sequencing, whereas ONT is rarely ...

A platform for highly parallel direct sequencing of native RNA strands was recently described by Oxford Nanopore Technologies, but despite initial efforts it remains crucial to further investigate the technology for quantification of complex transcriptomes. Now a team led by ...

Current technologies for targeted characterization and manipulation of viral RNA primarily involve amplification or ultracentrifugation with isopycnic gradients of viral particles to decrease host RNA background. The former strategy is non-compatible for characterizing properties innate to RNA strands such as ...

28 Feb 2019 at 11:00 (GMT -06:00) Jonathan Göke – Senior Research Scientist (PI) Genome Institute of Singapore (A*STAR) The ability of RNA-sequencing to generate a high-dimensional, quantitative readout...

Direct sequencing of RNA molecules such as virus genomes could help to unpick role of mysterious chemical modifications in genetic material. The genome of the flu virus has been fully sequenced in its native RNA form for the first time. ...

Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due...

A conference hosted by Oxford Nanopore Technologies. 24th – 25th May Old Billingsgate, London Delegate rates: NCM offer: £599* Limited rate: £699** Poster rate: £749** Standard rate: £999** Pre-meeting workshop: £400 The pre-meeting workshop is available to members of the ...

To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, researchers from McGill University sequenced cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix. This mix mimics mammalian mRNA species and consists of 92 polyadenylated transcripts ...

Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing — information ...