New Device Enables Low-Cost Single-Cell Analysis and Leads to Identification of Fibroblast Subtypes in Rheumatoid Arthritis Patients Single-cell analysis holds enormous potential to study how individual cells influence disease and respond to treatment, but the lack of cost-effective and user-friendly ...
Read More »Incorporation of unique molecular identifiers (UMIs) in TruSeq adapters improves the accuracy of quantitative sequencing
Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same genomic coordinates using reference-based alignment. ...
Read More »Using single-cell RNA sequencing to explore immune cell heterogeneity
Advances in single-cell RNA sequencing (scRNA-seq) have allowed for comprehensive analysis of the immune system. Here, researchers from New York University describe the available scRNA-seq technologies together with their corresponding strengths and weaknesses. They discuss in depth how scRNA-seq can be ...
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