Genomic datasets and the tools to analyze them have proliferated at an astonishing rate. However, such tools are often poorly integrated with each other: each program typically produces its own custom output in a variety of non-standard file formats. Here ...
Read More »Robust global microRNA expression profiling using next-generation sequencing technologies
miRNAs are a class of regulatory molecules involved in a wide range of cellular functions, including growth, development and apoptosis. Given their widespread roles in biological processes, understanding their patterns of expression in normal and diseased states will provide insights ...
Read More »Almost all steps of NGS library preparation protocols introduce bias, especially in the case of RNA-Seq
Next-generation sequencing (NGS) has caused a revolution in biology. NGS requires the preparation of libraries in which (fragments of) DNA or RNA molecules are fused with adapters followed by PCR amplification and sequencing. It is evident that robust library preparation ...
Read More »Next Generation Sequencing – Conference 2014
6-13 April 2014 Wellcome Trust Genome Campus, Hinxton, Cambridge Course summary Next-generation sequencing has become the premier tool in genetic and genomic analysis. This laboratory-based course is directed at scientists who will be generating and interpreting sequence information in their ...
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