Job Title: Bioinformatics Analyst Location: Chicago IL Department: MED-Biochem&Molecular Genetics Job Summary: The Department of Biochemistry and Molecular Genetics in the Feinberg School of Medicine is seeking Bioinformaticains to support current Next Generation Sequencing projects with datasets including ChIP-seq, Total ...
Read More »SHAPE-seq – reveals a nucleotide-resolution snapshot of RNA folding
By the time you reach the end of this sentence, RNA folding will have taken place in your body more than 10 quadrillion times. The folding of RNA is essential to life, yet because it happens so rapidly, researchers have ...
Read More »Post-doc position available – the ideal candidate would have substantial experience in RNA sequencing, and RNA isolation
Job Summary: The Auditory Regeneration Lab of the Department of Otolaryngology-Head and Neck Surgery, Northwestern University Feinberg School of Medicine has an immediate opening for a motivated postdoctoral fellow to work on regeneration of the auditory nerve. Our group is ...
Read More »Experimental approaches commonly used for single-cell gene expression profiling
Single-cell gene expression profiling was pioneered more than 20 years ago using exponential amplification of cDNAs by PCR and linear amplification by in vitro transcription. These protocols, as well as the subsequent availability of high-density microarrays, led to the application ...
Read More »Comparative evaluation of isoform-level gene expression estimation algorithms for RNA-seq and exon-array platforms
Given that the majority of multi-exon genes generate diverse functional products, it is important to evaluate expression at the isoform level. Previous studies have demonstrated strong gene-level correlations between RNA sequencing (RNA-seq) and microarray platforms, but have not studied their ...
Read More »Evaluation of data discretization methods to derive platform independent isoform expression signatures for multi-class tumor subtyping
Many supervised learning algorithms have been applied in deriving gene signatures for patient stratification from gene expression data. However, transferring the multi-gene signatures from one analytical platform to another without loss of classification accuracy is a major challenge. Here, researchers ...
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