Tutorial – High-Throughput Single-Cell RNA-Seq of Cells and Nuclei

October 2, 2017 Leave a comment 5,338 Views

Harnessing RNA-Seq to Create Gene Expression Profiles – from Genetic Engineering News – Jennifer Chew, Adam Bemis, Ronald Lebofsky, Ph.D., Anna Quinlan, Ph.D. , Kelly Kaihara, Ph.D. Complex biological systems rely on the coordinated activities of individual cells. The function of ...

A protocol for sequencing the transcriptome of a single nuclei

February 22, 2016 Leave a comment 6,882 Views

A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for ...

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Tag Archives: nuclei

Tutorial – High-Throughput Single-Cell RNA-Seq of Cells and Nuclei

A protocol for sequencing the transcriptome of a single nuclei

LACE-seq – global profiling of RNA-binding protein target sites

Researchers publish first transcriptomic study of decompression sickness

Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models

Bias in RNA-seq Library Preparation

Identifying differentially expressed circular RNAs associated with DNA methylation change

Single-cell RNA sequencing reveals how kidney cancer develops and responds to treatment

ppcseq – probabilistic outlier identification for RNA sequencing generalized linear models