Tutorial – High-Throughput Single-Cell RNA-Seq of Cells and Nuclei

October 2, 2017 Leave a comment 6,303 Views

Harnessing RNA-Seq to Create Gene Expression Profiles – from Genetic Engineering News – Jennifer Chew, Adam Bemis, Ronald Lebofsky, Ph.D., Anna Quinlan, Ph.D. , Kelly Kaihara, Ph.D. Complex biological systems rely on the coordinated activities of individual cells. The function of ...

A protocol for sequencing the transcriptome of a single nuclei

February 22, 2016 Leave a comment 7,711 Views

A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for ...

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Tag Archives: nuclei

Tutorial – High-Throughput Single-Cell RNA-Seq of Cells and Nuclei

A protocol for sequencing the transcriptome of a single nuclei

A comprehensive analytical protocol to interrogate multiple single-cell RNA-seq datasets with SingCellaR

scONE-seq – simultaneous single-cell transcriptome and genome profiling in a one-tube reaction

Planning for a giant leap: Workshop maps path toward RNA sequencing

New sequencing technique (SENT-seq) could accelerate and improve mRNA therapies

IgGenix demonstrates potential for single-cell RNA-sequencing in tree nut allergies

VASA-seq – high-throughput total RNA sequencing in single cells

ResPAN – a powerful batch correction model for scRNA-seq data through residual adversarial networks