Tutorial – High-Throughput Single-Cell RNA-Seq of Cells and Nuclei

October 2, 2017 Leave a comment 3,927 Views

Harnessing RNA-Seq to Create Gene Expression Profiles – from Genetic Engineering News – Jennifer Chew, Adam Bemis, Ronald Lebofsky, Ph.D., Anna Quinlan, Ph.D. , Kelly Kaihara, Ph.D. Complex biological systems rely on the coordinated activities of individual cells. The function of ...

A protocol for sequencing the transcriptome of a single nuclei

February 22, 2016 Leave a comment 5,547 Views

A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for ...

RNA-Seq Blog Transcriptome Research & Industry News

Tag Archives: nuclei

Tutorial – High-Throughput Single-Cell RNA-Seq of Cells and Nuclei

A protocol for sequencing the transcriptome of a single nuclei

Valid Post-clustering Differential Analysis for Single-Cell RNA-Seq

UCSF researchers recommend that all patients without Ras mutations undergo RNA sequencing to identify any fusions that might be treated with targeted therapies

Upcoming Live Chat Sessions: rRNA removal – The most underestimated step for RNA-seq optimization

Ribosomal RNA removal: The most underestimated step in RNA-seq optimization

Interactive Workshop – RNA-seq for Mendelian Diseases Diagnostics

A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes

A comparison of automatic cell identification methods for single-cell RNA sequencing data