Harnessing RNA-Seq to Create Gene Expression Profiles – from Genetic Engineering News – Jennifer Chew, Adam Bemis, Ronald Lebofsky, Ph.D., Anna Quinlan, Ph.D. , Kelly Kaihara, Ph.D. Complex biological systems rely on the coordinated activities of individual cells. The function of ...
Read More »A protocol for sequencing the transcriptome of a single nuclei
A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for ...
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