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  • New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens
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  • Long term and short-term effects of RNA fixation on Single Cell RNA Sequencing
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Tag Archives: Nudipleura

Webinar Today – Using Next-Gen Sequencing to Study the Evolution of Neural Circuitry

April 30, 2015 Leave a comment 2,338 Views

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The April 30, 2015 Webinar will look at the evolution of behavior at the levels of single neurons, neural circuits, and their synaptic connections as it is related to the swimming behavior of sea slugs. Adriano Senatore, PhD, MSc, BSc ...

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Recent RNA-SEQ Pubs

Transcriptomic analysis provides insights into candidate genes and molecular pathways involved in growth of Manila clam Ruditapes philippinarum
4 March 2021
De novo assembly of the transcriptome of scleractinian coral, Anomastraea irregularis and analyses of its response to thermal stress
4 March 2021
Nuclear RNA purification by flow cytometry to study nuclear processes in plants
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Co-expression of glutamatergic and autism-related genes in the hippocampus of male mice with disturbances of social behavior
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Data of de novo transcriptome assembly of the myxozoan parasite <em>Tetracapsuloides bryosalmonae</em>
4 March 2021
Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells
4 March 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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  • rna-seq

    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    19 hours ago
  • rna-seq

    RNA sequencing brings hope for endangered wild cats

    20 hours ago
  • rna-seq

    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

    2 days ago
  • rna-seq

    Uncovering the Anti-Myeloma Resistance Files

    2 days ago
  • Long term and short-term effects of RNA fixation on Single Cell RNA Sequencing

    3 days ago
  • rna-seq

    Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source

    3 days ago
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    2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

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